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. 2020 Apr 2;19:1536012120913693. doi: 10.1177/1536012120913693

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Quantification of signal and noise in vitro. A, Quantification of trastuzumab binding to SKBR3 (HER2+) cells shows average of 30 to 50 000 antibodies/μm², while S1 (HER2-) cells are ×17 less. B, Cell staining of SKBR3 and (C) cell staining of S1 with trastuzumab (green = trastuzumab, blue = nucleus) with various concentrations of anti-HER2 antibody. D-F, Similar experiment with prostate cancer cell line LNCaP (PSMA+) and PC3 (PSMA-). D, Quantification of J591 binding to LNCaP cells shows 40 000 antibodies/μm². (E and F) Cell staining with J591 (green = J591, blue = nucleus) with various concentrations of J591 with LNCaP and PC3, respectively.