Table 3.

Temperatures recorded with a heating stage at 37°C, without air laminar flow

Group	Time
	Onset	3 minutes	5 minutes	10 minutes
1-37	36.98 ± 0.01A	35.92 ± 0.07	35.62 ± 0.17*	35.38 ± 0.20*
2-37	36.99 ± 0.01A	35.12 ± 0.16	34.61 ± 0.24B	33.53 ± 0.12
3-37	36.99 ± 0.01A	34.83 ± 0.26*	34.48 ± 0.42B*	33.80 ± 0.05
4-37	36.98 ± 0.01A	30.79 ± 0.15	29.48 ± 0.21B	28.37 ± 0.10

Data are presented as mean ± SD. In each type of culture dish, the temperature differences recorded at different time points were statistically significant except the cases denoted with *(Sidak’s test). In pair-wise comparisons between groups (Tukey’s test) all differences were statistically significant except the cases denoted with the same capital letters. Group 1-37; 50 mm ICSI dishes with 5 droplets of culture medium (4 μl each) and a line of 10 μl PVP, with 4 ml of oil, Group 2-37; 35 mm dishes with 5 droplets of 50 μl culture medium, covered with 4 ml of light mineral oil, Group 3-37; 60 mm dishes with 5 droplets of 50 μl culture medium, covered with 12 ml of light mineral oil, Group 4-37; 90 mm dishes containing 14 ml of culture medium, without oil.

ICSI; Intracytoplasmic sperm injection and PVP; Polyvinyl pyrrolidone.