Table 4.
Temperatures recorded with heating stage at 37°C and air laminar flow
| Group | Time | |||
|---|---|---|---|---|
| Onset | 3 minutes | 5 minutes | 10 minutes | |
| 1 air 37 | 36.98 ± 0.01A | 34.68 ± 0.08 | 34.48 ± 0.15 | 34.18 ± 0.10 |
| 2 air 37 | 36.98 ± 0.01A | 33.91 ± 0.07 | 33.23 ± 0.07 | 32.77 ± 0.07 |
| 3 air 37 | 36.99 ± 0.01A | 34.46 ± 0.08 | 33.79 ± 0.07 | 32.94 ± 0.02 |
| 4 air 37 | 36.98 ± 0.01A | 27.78 ± 0.20 | 26.42 ± 0.22 | 25.34 ± 0.20 |
Data are presented as mean ± SD. In each type of culture dish, the temperature differences recorded at different time points were statistically significant (Sidak’s test). In pair-wise comparisons between groups (Tukey’s test) all differences were statistically significant except the cases denoted with the same capital letters. Group 1 air 37; 50 mm ICSI dishes with 5 droplets of culture medium (4 μl each) and a line of 10 μl PVP, with 4 ml of oil, Group 2 air 37; 35 mm dishes with 5 droplets of 50 μl culture medium, covered with 4 ml of light mineral oil, Group 3 air 37; 60 mm dishes with 5 droplets of 50 μl culture medium, covered with 12 ml of light mineral oil, Group 4 air 37; 90 mm dishes containing 14 ml of culture medium, without oil. ICSI; Intracytoplasmic sperm injection and PVP; Polyvinyl pyrrolidone.