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. 2020 Feb 25;14(1):63–67. doi: 10.22074/ijfs.2020.5917

Table 4.

Temperatures recorded with heating stage at 37°C and air laminar flow


Group Time
Onset 3 minutes 5 minutes 10 minutes

1 air 37 36.98 ± 0.01A 34.68 ± 0.08 34.48 ± 0.15 34.18 ± 0.10
2 air 37 36.98 ± 0.01A 33.91 ± 0.07 33.23 ± 0.07 32.77 ± 0.07
3 air 37 36.99 ± 0.01A 34.46 ± 0.08 33.79 ± 0.07 32.94 ± 0.02
4 air 37 36.98 ± 0.01A 27.78 ± 0.20 26.42 ± 0.22 25.34 ± 0.20

Data are presented as mean ± SD. In each type of culture dish, the temperature differences recorded at different time points were statistically significant (Sidak’s test). In pair-wise comparisons between groups (Tukey’s test) all differences were statistically significant except the cases denoted with the same capital letters. Group 1 air 37; 50 mm ICSI dishes with 5 droplets of culture medium (4 μl each) and a line of 10 μl PVP, with 4 ml of oil, Group 2 air 37; 35 mm dishes with 5 droplets of 50 μl culture medium, covered with 4 ml of light mineral oil, Group 3 air 37; 60 mm dishes with 5 droplets of 50 μl culture medium, covered with 12 ml of light mineral oil, Group 4 air 37; 90 mm dishes containing 14 ml of culture medium, without oil. ICSI; Intracytoplasmic sperm injection and PVP; Polyvinyl pyrrolidone.