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Effect of OXY on mRNA and protein expression of MUC2. Cells were treated with OXY (10 μg/mL) with or without the autophagy inhibitor 3-MA. The expression level of MUC2 was measured by qPCR (A). The expression level of the MUC2 protein was determined by ICC (B) and quantified (C). Each value indicates the mean ± SD of three independent experiments performed in triplicate.
