Table 1.
Primers used for qPCR analysis.
| Gene | Forward (5′ to 3′) | Reverse (5′ to 3′) |
|---|---|---|
| GAPDH | GAGTCAACGGATTTGGTCGT | GACAAGCTTCCCGTTCTCAG |
| ULK1 | CAGAACTACCAGCGCATTGA | TCCACCCAGAGACATCTTCC |
| ULK2 | GTGGGAACGTGAGGAAGAGG | CTGGGAGTTTCAGGGTGGTC |
| Atg13 | AAAGTCACCTCCCAGTGTGG | GAAAAGCTCCACAGGACAGC |
| Atg101 | GAAGTGTGGACGGTCAAGGT | CACGTTATCCACCTCCGACT |
| VPS34 | AAGCAGTGCCTGTAGGAGGA | TGTCGATGAGCTTTGGTGAG |
| Beclin-1 | AGGTTGAGAAAGGCGAGACA | AATTGTGAGGACACCCAAGC |
| Atg7 | GGCCTAGGTACCTGTGACCA | GAAGCTCCCATGAGCTGAAC |
| Atg4 | GGCTTACCAAGGGCTACCTC | ACAGCACTGGAAAGGGACAC |
| Atg5 | ACCAGAAACACTTCGCTGCT | GACCTTCAGTGGTCCGGTAA |
| Atg16L1 | CACAAGAAACGTGGGGAGTT | ACAAAGCTTAGTGCGCAGGT |
| LC3B | CCACACCCAAAGTCCTCACT | CACTGCTGCTTTCCGTAACA |
| Rab7 | TGGATGACAGGCTAGTCACG | CTGGCCTGGATGAGAAACTC |
| STX17 | GGGTGAAGCCAGGAATGTTA | ATGCCACACCCAGCTAATTC |
| VAMP8 | CATCTCCGCAACAAGACAGA | GACCCTCTTGGCACACATTT |
| LAMP2 | ACAACTCACTCCACAGGCAG | TGCAATGCTGAAAACGGAGC |
| MEK | GCTTGGGGCTATTTGTGTGT | TCTCACAAGGCTCCCTCCTA |
| ERK1 | TCA GAC TCC AAA GCC CTT GAC | CGT GCT GTC TCC TGG AAG ATG |
| ERK2 | TCC AAC AGG CCC ATC TTT CC | CCA GAG CTT TGG AGT CAG CA |
| IRE1α | CGGCCTTTGCAGATAGTCTC | ACGTCCCCAGATTCACTGTC |
| XBP1 | GGAGTTAAGACAGCGCTTGG | ACTGGGTCCAAGTTGTCCAG |
| PERK | CTCACAGGCAAAGGAAGGAG | AACAACTCCAAAGCCACCAC |
| ATF4 | TCAAACCTCATGGGTTCTCC | GAAGGTCATCTGGCATGGTT |
| MUC2 | ACCCGCACTATGTCACCTTC | GGACAGGACACCTTGTCGTT |