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. 2020 Mar 18;21(6):2083. doi: 10.3390/ijms21062083

Figure 5.

Figure 5

Increased susceptibility to cucumber mosaic virus (CMV) infection in LreEF1A4-overexpressing transgenic petunia plants. (A) Disease symptoms of wild-type (WT) and transgenic lines (1B, 2A, and 8E) at 14 days post inoculation (DPI) with CMV. The plantlets at the four-leaf stage were used for viral inoculation. The necrotic lesions caused by CMV multiplication are marked in dashed circles. Scale bars = 5.0 mm. Number (B) and relative area (C) of necrotic lesions in systemically-infected leaves of WT and transgenic lines at 14 DPI. (D) Quantitative real-time polymerase chain reaction (PCR) analysis of CMV-CP transcripts encoding CMV coat protein in uppermost younger leaves of petunia plants at 10 and 14 DPI. 26S rRNA was used as an internal standard. Error bars represent standard error (SE) of the mean from three independent biological replicates. Asterisks indicate statistical significance as calculated by Student’s t-test at p < 0.05.