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. 2020 Mar 18;21(6):2083. doi: 10.3390/ijms21062083

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Increased accumulation of tobacco rattle virus-green fluorescent protein (TRV-GFP) in LreEF1A4-overexpressing transgenic petunia plants. GFP fluorescence (A) and relative fluorescent area (B) in TRV-GFP-inoculated leaves of wild-type (WT) and transgenic lines (1B, 2A, and 8E). Photographs were taken at 4 days post inoculation (DPI). Scale bars = 5.0 mm. (C) Quantitative real-time polymerase chain reaction (PCR) analysis of TRV RNA1 and RNA2 accumulation levels in the leaves of WT and transgenic petunia plants at 4 DPI. Abundance of 26S rRNA transcripts was used as an internal control. Error bars represent standard error (SE) of the mean from three independent biological replicates. Significance of difference was evaluated via Student’s t-test (p < 0.05) and denoted by asterisks.