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. 2020 Mar 18;21(6):2083. doi: 10.3390/ijms21062083

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Enhanced tolerance to salt and drought stresses in transgenic petunia plants overexpressing LreEF1A4. (A) Representative phenotypes of wild-type (WT) and transgenic petunia seedlings (1B, 2A, and 8E) grown in Murashige and Skoog (MS) media containing 0 and 200 mM NaCl. Photographs were taken at 10 and 20 days after sowing (DAS). Scale bars = 1.0 cm. (B) Germination percentage of WT and transgenic seedlings at 20 DAS. Representative phenotypes (C) and total fresh weight (D) of WT and transgenic petunia seedlings at 5 days post treatment with dehydration. Five-week-old plantlets were used for the treatment. Scale bars = 1.0 cm. Error bars represent standard error (SE) of the mean from three independent biological replicates. Asterisks denote statistical significance as determined by Student’s t-test (p < 0.05).