Figure 1.

SHIP2 physically interacts with IQGAP2 in GC cells. (A,B) Interaction between SHIP2 and IQGAP2 was detected by co-immunoprecipitation (co-IP) with anti-SHIP2 or anti-IQGAP2 antibodies in SGC-7901 and MGC-803. The Immunoglobulin G (IgG) group acted as negative control; (C) Co-localization of SHIP2 and IQGAP2. MGC-803 cells were immunostained with anti-SHIP2 and anti-IQGAP2 antibodies followed by incubation with Alexa-488 conjugated anti-rabbit antibodies and Alexa-594 conjugated anti-mouse antibodies, respectively. Images were taken with a confocal microscope. Data shown are representative of three individual experiments.