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. 2020 Mar 13;21(6):1968. doi: 10.3390/ijms21061968

Figure 3.

Figure 3

IQGAP2 inhibits the migration and invasion of GC cells in association with SHIP2 phosphatase activity. (A) Whole-cell lysates from stable SGC-7901 and MGC-803 cells carrying a lentivirus-mediated IQGAP2 or SHIP2 shRNA, and their corresponding counterparts were subjected to Western blot analysis for the indicated proteins. Data shown are representative of three individual experiments; (B) SHIP2 proteins, immunoprecipitated by anti-SHIP2 antibodies in stable SGC-7901 and MGC-803 cells carrying a lentivirus-mediated IQGAP2 or SHIP2 shRNA, were used to detect its phosphatase activity by malachite green phosphatase assays (n = 3, mean ± SEM, * p < 0.05); (C) the migration of SGC-7901 cells carrying a lentivirus-mediated IQGAP2 or SHIP2 shRNA was detected by wound-healing assays. Right panel shows quantitation of wound closure corresponding to the left panel. The y axis represents the percentages of wound closure at 24 or 48 h after wound introduction (n = 3, mean ± SEM, * p < 0.05); (D,E) Migration and invasion of SGC-7901 and BGC-823 cells carrying a lentivirus-mediated IQGAP2 or SHIP2 shRNA were detected by Transwell assays (n = 3, mean ± SEM, * p < 0.05). Magnification, ×200.