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. 2020 Mar 13;21(6):1968. doi: 10.3390/ijms21061968

Figure 4.

Figure 4

The inhibition of IQGAP2 on the migration and invasion of GC cells is partially due to elevating SHIP2 phosphatase activity. (A) Whole-cell lysates from stable SGC-7901.SHIP2 and SGC-7901.NC cells transduced with IQGAP2 or scramble shRNA were subjected to Western blot analysis for the indicated proteins. Data shown are representative of three individual experiments; (B) SHIP2 proteins, immunoprecipitated with anti-SHIP2 antibodies in stable SGC-7901.SHIP2 and SGC-7901.NC cells transduced with IQGAP2 or scramble shRNA, were used to detect its phosphatase activity by malachite green phosphatase assays (n = 3, mean ± SEM, * p < 0.05); (C) the migration of stable SGC-7901.SHIP2 and SGC-7901.NC cells transduced with IQGAP2 or scramble shRNA was detected by wound-healing assays. Right panel shows quantitation of wound closure corresponding to the left panel. The y axis represents the percentages of wound closure at 24 or 48 h after wound introduction (n = 3, mean ± SEM, * p < 0.05); (D,E) Migration and invasion of stable SGC-7901.SHIP2 and SGC-7901.NC cells transduced with IQGAP2 or scramble shRNA were detected by Transwell assays (n = 3, mean ± SEM, * p < 0.05). Magnification, ×200.