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. 2020 Mar 13;21(6):1968. doi: 10.3390/ijms21061968

Figure 5.

Figure 5

Binding to PRD and SAM domains of SHIP2 is crucial for the inhibitory effects of IQGAP2 on the migration and invasion of GC cells. (A) Whole-cell lysates from stable SGC-7901.shSHIP2 cells transfected with wild-type SHIP2 and deletion mutant SHIP2Δ935-1258 plasmids were subjected to Western blot analysis for the indicated proteins. Data shown are representative of three individual experiments; (B) SHIP2 proteins, immunoprecipitated by anti-SHIP2 antibodies in stable SGC-7901.shSHIP2 cells (transfected with wild type SHIP2 and deletion mutant SHIP2Δ935-1258 plasmids), were used to detect its phosphatase activity by malachite green phosphatase assays (n = 3, mean ± SEM, * p < 0.05); (C,D) the migration and invasion of stable SGC-7901.shSHIP2 cells transfected with wild-type SHIP2 and deletion mutant SHIP2Δ935-1258 plasmids were detected by Transwell assays (n = 3, mean ± SEM, * p < 0.05). Magnification, ×200.