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Comparison of physical binding sites identified by gDB-seq analysis among bZIP TFs. (A) Detection of recombinant proteins with an HA-tag at the N-terminal by SDS-PAGE gel electrophoresis. Recombinant proteins were synthesized in wheat germ extracts. (B) Overlap between genes with promoters possessing HY5-binding sites and genes with promoters possessing binding sites of HYH, GBF1, GBF2 or GBF3. (C) Nucleotide numbers are relative to the translation initiation site (TIS) (+1).
