Table 3.

Comparison between iPSC-derived 2D systems and 3D organoid models.

	2D Systems	3D Organoids
Culture method	- cell growth and differentiation on monolayers	- cell differentiation and self-organization within matrigel
Cell population	- usually immature cell populations	- improved maturation
Duration of differentiation	- fast differentiation process	- slow differentiation process
Tissue composition	- lack of tissue microenvironment	- similar cytoarchitecture with in vivo tissue
Vascular supply	- no	- limited
High-throughput generation	- high	- low
Genome editing	- easy	- hard
Technical procedure	- mostly easy - less time consuming	- moderate - more time consuming
Disease modeling specificity	- moderate	- high