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. 2020 Feb 28;9(3):200. doi: 10.3390/antiox9030200

Figure 4.

Figure 4

The effect of CJ extract on the growth factors and protective effect against oxidative stress of C. elegans. CJ extracts (25 and 50 μg/mL) does not change normal growth factors: (A) population, (B) progeny production. (C) CJ extracts (25 and 50 μg/mL) delayed the age-related behavioral symptom: pumping speed. Since the L1 stage, the nematodes were treated with 25 and 50 μg/mL CJ extracts for measuring normal growth factors and pumping speed. Each value represents mean ± standard error (n = 3 plates and 100 worms/plate for growth rate; n = 3 plates and five worms/plate for progeny production; n = 3 plates and 15 worms/plate for pumping rate) (D) CJ extracts (25 and 50 μg/mL) lowered ROS generation in C. elegans. Each bar represents the mean ± SD of three individual experimental results (n = 3). Letters above bars indicate significant differences according to Duncan’s multiple range test (p < 0.05).