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. 2020 Mar 15;21(6):2003. doi: 10.3390/ijms21062003

Figure 1.

Figure 1

Construction of polymorphic membrane protein D N-terminal pEGFP-(pmpD-N) plasmids. (A) The pmpD-N gene was cloned and identified to product 116 bp by the PCR method. M: Marker (MD103); 1: Negative control; 2: pmpD-N gene. (B) Construction of pEGFP-N1-pmpD-N plasmids. (C) The pmpD-N genes were expressed in HD cells and identified by RT-PCR assay. M: Marker (MD103); 1: RNA product of pEGFP-N1-pmpD-N plasmid transfected into HD11 cells; 2: DNA product of pEGFP-N1 plasmid transfected into HD11 cells. (D) The PmpD-N proteins were detected by Western blotting assay.