Figure 4.

mRNA expression of Toll-like receptors (TLRs), myeloid differentiation factor 88 (MyD88), and nuclear factor kappa B (NF-κB) post-treatment with PmpD-N or pEGFP-N1-pmpD-N. The mRNA expression levels of TLR1–TLR5, MyD88, and NF-κB were determined by qRT- PCR. At different time points, the cell cultures were quantified in triplicate by qRT-PCR for levels of mRNA expression of TLRs and MyD88 by antigen-stimulated and C.-psittaci-infected HD11 cells (A) 6 h; (B).12 h; (C).24 h; (D).48 h. Statistical analyses were performed with one-way analysis of variance (ANOVA) with Tukey’s post hoc multiple comparisons test (** p < 0.01 when compared with the plasmid control group).