Figure 7.

Effect of short interfering RNA (siRNA) or treatment with NF-κB inhibitor on cytokine secretions in HD11 cells. HD11 cells were transfected with siRNA targeting TLR2, TLR4, and MyD88 or treated with the NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC) before stimulation with PmpD-N. Cell supernatants were collected and assayed for secretions of (A) IL-6 and (B) IL-10 using ELISA kits. Each experiment was repeated at least twice. Data are shown as the mean + SD values for triplicate cultures for one of two independent experiments. Statistically significant differences among different groups were evaluated with one-way analysis of variance (ANOVA) with Tukey’s post hoc multiple comparisons test and were considered to be statistically significant at ** p < 0.01.