Figure 8.

Effect of siRNA or treatment with the NF-κB inhibitor on phagocytic function and NO production in HD11 cells. HD11 cells were transfected with siRNA targeting TLR2, TLR4, and MyD88 or treated with the NF-κB inhibitor PDTC before stimulation with PmpD-N. Phagocytic (A) function and NO (B) production were determined in cell supernatants. Each experiment was repeated at least twice. Data are shown as the mean ± SD values for triplicate cultures for one of two independent experiments. Statistically significant differences among different groups were analyzed with one-way analysis of variance (ANOVA) with Tukey’s post hoc multiple comparisons test.