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. 2020 Mar 2;12(3):575. doi: 10.3390/cancers12030575

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Xenotransplanted tumors produce WNK1 and induce endogenous WNK1 in zebrafish. (A) QPCR analysis of human WNK1 expression in xenotransplanted hepatoma at 0–3 dpi. (B,C) QPCR analysis of endogenous wnk1a and wnk1b in zebrafish 0–3 dpi hepatoma xenotransplantation. Statistical significance relative to 0 dpi. The statistical significance was analyzed by unpaired Student’s t-test. ns: non-significant, p > 0.05; *: 0.01 < p ≤ 0.05; **: 0.001 < p ≤ 0.01; ***: 0.0001 < p ≤ 0.001; ****: p ≤ 0.0001.