Table 1.
The primer information for qPCR analysis in human cell line and zebrafish.
| Gene Name | Primer Name | Sequence (5′ to 3′) |
|---|---|---|
| VEGFA | Q-hVEGFA-F | GGGCAGAATCATCACGAAGT |
| Q-hVEGFA-R | TGGTGATGTTGGACTCCTCA | |
| WNK1 | Q-hWNK1-F | GAGCGTCATCTGTGACTCCA |
| Q-hWNK1-R | CGGTCTTTGCTGGTACTGCT | |
|
EGFP
Standard Curve |
Q-EGFP-N1-F | ACGTAAACGGCCACAAGTTC |
| Q-EGFP-N1-R | AAGTCGTGCTGCTTCATGTG | |
| pEGFP-N1-F | CCTGATTCTGTGGATAACCGTAT | |
| pEGFP-N1-R | TGCATTCTAGTTGTGGTTTGTCC | |
| RPIA | Q-RPIA-F | CATGCTGTGCAGCGAATAGC |
| Q-RPIA-R | TGGCGGGCCTGGAAGGAAGT | |
| ccnd1 | Q-ccnd1-F | TTGCCTCTCATCCCAGAACCT |
| Q-ccnd1-R | CCTGACACGATCGCAGACAGT | |
| wnk1a | Q-wnk1a-F | TCGAGATAGGACGTGGCTCT |
| Q-wnk1a-R | TCAAGGATGATTCCCAGGAG | |
| wnk1b | Q-wnk1b-F | CCGGGTCAGCTGTGCCCAAG |
| Q-wnk1b-R | TGGCCCAGGGGTTGTGAGGT | |
| mmp9 | Q-mmp9-F | GGTGATTGACGACGCCTTTG |
| Q-mmp9-R | GAAATGGGCGTCTCCCTGAA | |
| flk-1 | Q-flk1-F | TCTTCACTCTTCACGTGCTTTTTAG |
| Q-flk1-R | GAAGGTGTGTATCTCCATCAGGAA | |
| hpse | Q-hpse-F | GCTCTGGTTTGGAGCTCATC |
| Q-hpse-R | GAAATCCCGACCAAGTTGAA | |
| chrebp1 | Q-chrebp-F | GGAGATGGACTCGCTCTTTG |
| Q-chrebp-R | GCAGAGGCTCAAAAGTGTCC | |
| actin | Q-actin-F | CTCCATCATGAAGTGCGACGT |
| Q-actin-R | CAGACGGAGTATTTGCGCTCA |