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. 2020 Mar 19;21(6):2124. doi: 10.3390/ijms21062124

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Western Blot results. (A) Expression levels of Sirtuin 1 (SIRT1) in the different groups of the study. (B) Expression levels of Matrix metalloproteinase 9 (MMP9) in the different groups. Detected proteins: Histone H3 (~17kDa), SIRT1 (~120kDa), beta-actin (~42kDa), MMP9 (~92kDa). All data are presented as group mean ± standard error of the mean (SEM); n = 6 animals in each group. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns = non-significant. Statistical analysis was done using GraphPad Prism: after estimation of Gaussian distribution with Shapiro-Wilk normality test data was either analysed with one-way analysis of variance (ANOVA) or non-parametric Kruskal-Wallis test.