Table 1.
List of antibodies employed in this study.
| Target Protein 1 | Species | Catalog No. | [WB] 2 | [IIF] 3 |
|---|---|---|---|---|
| α-Tubulin * | Mouse | Abcam, ab7291 | 1:4000 | - |
| ARL2 | Rabbit | Abcam; ab183510 | 1:5000 | - |
| BUB3 | Rabbit | Abcam; ab4180 | 1:5000 | - |
| CCNE1 | Rabbit | Abcam; ab133266 | 1:5000 | 1:200 |
| Cyclophilin B * | Rabbit | Abcam; ab16045 | 1:50,000 | - |
| DSN1 | Rabbit | Thermo Fisher; PA534879 | 1:1000 | - |
| GARS | Rabbit | Abcam; ab42905 | 1:5000 | - |
| GART | Rabbit | Abcam; ab169550 | 1:5000 | - |
| γH2AX | Rabbit | Abcam; ab2893 | - | 1:1000 |
| NUF2 | Rabbit | Abcam; ab176556 | 1:20,000 | - |
| PIGS | Rabbit | Abcam; ab113817 | 1:5000 | - |
| RPA32/RPA2 | Mouse | Abcam; ab2175 | - | 1:200 |
| SHMT2 | Rabbit | Abcam; ab180786 | 1:1000 | - |
| SKP1 | Mouse | Abcam; ab124473 | 1:5000 | - |
| SPC24 | Rabbit | Abcam; ab157184 | 1:5000 | - |
| Anti-Rabbit HRP | Goat | Jackson Immunoresearch; 111-035-114 | 1:10,000 | - |
| Anti-Mouse HRP | Goat | Jackson Immunoresearch; 115-035-146 | 1:10,000 | - |
| Anti-Rabbit AlexFluor488 | Goat | Abcam; ab150081 | - | 1:200 |
| Anti-Mouse Cy3 | Goat | Abcam; ab150117 | - | 1:200 |
1 Proteins listed with an asterisk represent Western blot loading controls. HRP = horse radish peroxidase. 2 Concentration of antibody employed for Western blot analyses. 3 Concentration of antibody employed for indirect immunofluorescence. * identifies protein loading controls.