Figure 1.

Phase contrast images of C2C12 cells after 4 and 24 hours of exposure to conditioned media (CM) derived from Single clone pick 1 (SCP1)^GFP or SCP1^GFP/SCX cells led to smaller cells with fewer filopodia (arrowheads) when compared to myoblasts in normal medium (NM) (A). Quantification of the individual cell morphology parameters revealed a significant decrease in cell area (B) but not of the aspect ratio (C). Long-term treatment (24 h) significantly increased the metabolic activity of C2C12 in CM of SCP1^GFP or SCP1^GFP/SCX cells, when compared to myoblasts in normal medium. Interestingly, the secretome of scleraxis-overexpressing cells led to a higher metabolic activity of C2C12 cells, when compared to CM from SCP1^GFP (D). Scale bar: 100 µm. Violin plots represent the median (red line) and quartiles (black lines). Bar plots represent the mean and SD. * equals p ≤ 0.05, ** equals p ≤ 0.01, *** equals p ≤ 0.001, n.s.: not significant. Data were obtained from three randomly selected field-of-views (FOV) of three independent experiments. At least 75 cells per time point and experimental condition were evaluated.