Figure 5.

Macrophage-secreted iron induces proliferation and migration of tumor cells in vitro. (A) Schematic overview of how to generate conditioned medium from iron-releasing human MΦ. (B) Iron amount measured by AAS relative to the total protein amount in the supernatant of primary human MΦ, either left untreated (ctrl) or stimulated with IL-10 (20 ng/mL; 24 h) (n = 5). Proliferation of (C) CAKI 1 (n = 4) and (D) 786-O (n = 4) cells as well as migration of (E) CAKI 1 (n = 4) and (F) 786-O cells (n = 4) upon stimulation with the supernatant of IL-10-stimulated MΦ in the presence or absence of an extracellular chelator (EC1, 100 µM) or dimethyl sulfoxide (DMSO) as negative control measured with the xCELLigence system. Graphs are displayed as means ± SEM with * p < 0.05, ** p < 0.01, *** p < 0.001.