Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 25;12(3):530. doi: 10.3390/cancers12030530

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Tumor-associated MΦ express enhanced FPN protein. (A) FACS panel how to gate CD45⁺ immune cells and CD326⁺ epithelial/tumor cells. Immune cells were further gated for CD33⁺/HLA-DR⁺ cells of which CD64⁺ and MerTK⁺ MΦ were sub-selected. Cells were subsequently analyzed for their FPN protein expression, displayed as MFI (mean fluorescence intensity). (B) FPN protein expression as MFI of CD45^-/CD326^- stroma cells (n = 26). (C) FPN protein expression as MFI of MΦ in tumor tissue compared to adjacent healthy tissue (n = 26). (D) Representative pictures for the MΦ marker CD163 and FPN protein expression in tumor tissue compared to healthy adjacent tissue applying confocal laser scanning miscroscopy. DAPI was used as nuclear stain. Scale bar: 200 µm. FPN mRNA expression normalized to 18S expression correlated to (E) low (G1–G2) and high (G3–G4) tumor grade and (F) low (pT1–pT2) and high (pT3–pT4) tumor pT-stage in our patient cohort (upper panels; n = 48) compared to the TCGA data base, applying the ccRCC-KIRC data set (lower panels; n = 70). (G) Kaplan-Meier curve of high or low FPN expression from the R2 bioinformatics platform using the ccRCC-KIRC data set. Graphs are displayed as means ± SEM with * p < 0.05, ** p < 0.01, *** p < 0.001.

Tumor-associated MΦ express enhanced FPN protein. (A) FACS panel how to gate CD45⁺ immune cells and CD326⁺ epithelial/tumor cells. Immune cells were further gated for CD33⁺/HLA-DR⁺ cells of which CD64⁺ and MerTK⁺ MΦ were sub-selected. Cells were subsequently analyzed for their FPN protein expression, displayed as MFI (mean fluorescence intensity). (B) FPN protein expression as MFI of CD45^-/CD326^- stroma cells (n = 26). (C) FPN protein expression as MFI of MΦ in tumor tissue compared to adjacent healthy tissue (n = 26). (D) Representative pictures for the MΦ marker CD163 and FPN protein expression in tumor tissue compared to healthy adjacent tissue applying confocal laser scanning miscroscopy. DAPI was used as nuclear stain. Scale bar: 200 µm. FPN mRNA expression normalized to 18S expression correlated to (E) low (G1–G2) and high (G3–G4) tumor grade and (F) low (pT1–pT2) and high (pT3–pT4) tumor pT-stage in our patient cohort (upper panels; n = 48) compared to the TCGA data base, applying the ccRCC-KIRC data set (lower panels; n = 70). (G) Kaplan-Meier curve of high or low FPN expression from the R2 bioinformatics platform using the ccRCC-KIRC data set. Graphs are displayed as means ± SEM with * p < 0.05, ** p < 0.01, *** p < 0.001.