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. 2020 Mar 19;7(2):ENEURO.0320-19.2020. doi: 10.1523/ENEURO.0320-19.2020

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Copyright © 2020 Talla and Koilkonda

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 5. — Axonal regeneration: immunoblotting of (A) ON and (B) retina samples obtained from 18 MPS/9 MPI of control-Veh-ttd, EAE-Veh-ttd, and EAE-Klf4^fl/fl-Cre-ttd mice shows expression of GAP43 (top panels) and GAPDH (bottom panels) in all samples. GAP43 band intensities were higher in EAE-Klf4^fl/fl-Cre-ttd mice compared with control or EAE Veh-ttd mice. Corresponding box-whisker plots at the bottom of the blots shows the ImageJ-based quantitative analysis of GAP43/GAPDH expression in ON and retina among three groups. Samples derived from the same experiment and that gels/blots were processed in parallel for quantitative analysis. Statistical analysis was performed by one-way ANOVA followed by Tukey’s multiple comparison test, a p < 0.05 is considered to be statistically significant; ****p < 0.0001–0.0009 (n = 6 eyes/group, experimental repeats = 3).Figure Contributions: Venu Talla acquired the data, analyzed, and generated the figure.