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. 2020 Mar 7;12(3):623. doi: 10.3390/cancers12030623

Figure 4.

Figure 4

Lung fluorescence images after injection of 67NR/iRFP and 4T1/iRFP cells preceded by RBP4 protein administration. (A) 67NR/iRFP or (B) 4T1/iRFP cells were inoculated intravenously (i.v.). Three (-3 h) or one (-1 h) hour before cell inoculation, mice were administered i.v. with 500 ng/mouse of RBP4 or with saline. Alternatively, the cells were incubated with 200 ng/mL of RBP4 for 24 h and then inoculated i.v. (24 h preincubation). The lungs from healthy mice inoculated i.v. with saline were used as a reference for fluorescence measurements. Healthy mice injected with 500 ng/mouse of RBP4 were used as an additional control (RBP4). Lung fluorescence measurements were performed 48 h after cell inoculation. (C) Fluorescence of representative lungs from all groups is presented. No. of mice per group: healthy and RBP4 = 5, -3 h control = 6, remaining groups = 8–10. Data presented as mean ± SD. Statistical analysis: Unpaired t-test * p < 0.05, ** p < 0.01, *** p < 0.001.