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. 2020 Mar 7;12(3):623. doi: 10.3390/cancers12030623

Figure 6.

Figure 6

In vitro characteristics of the effect of RBP4 on 4T1 and 67NR mouse mammary gland cancer. (A) Comparison of RBP4 level in 4T1 and 67NR cell lysates using ELISA. (B–D) The effect of 24 h incubation with 200 ng/mL of RBP4 on (B) cell proliferation (N = 4), (C) migration (N = 6), and (D) adhesion (N = 3). (E) The proliferation of wild-type and transduced 67NR cells measured between 24 and 144 h (N = 2–6). (F) Number of colonies formed 14 days after seeding three different numbers of wild-type and transduced 67NR cells (N = 3 for 150 cells; N = 8 for 50 and 100 cells/well). (G) Representative image of colonies formed after seeding of 100 cells/well. (H) Migration of 67NR/0 and 67NR/RBP4 cell lines through collagen and fibronectin (N = 4–6). (I) The sensitivity of 67NR/RBP4 cells to commonly used anticancer drugs (N = 3–7). Statistical analysis: Tukey’s multiple comparison test or unpaired t test; (I) Dunnett’s multiple comparison test. * p < 0.05, ** p < 0.01, **** p < 0.0001.