Table 1.
Cell Line | Route of Transplantation | Age of Mice (weeks) | Time Points of Euthanasia—Days after Cell Transplantation |
---|---|---|---|
67NR, 4T1 | ort. | 6 | 12, 24, 33, “26” |
67NR, 4T1 | ort. | 52 | 28 (4, 9, 15, 21) * |
67NR, 67NR/0, 67NR/RBP4, 4T1, 4T1/0, 4T1/RBP4 | ort. | 16 | 12, 24, 33 **, “26” |
67NR/iRFP and 4T1/iRFP | i.v. | 16 | 48 h after cell transplantation |
* in brackets: days of blood collection from zygomatic vein; ** 67NR/0 cell line: last time point of euthanasia 43 day; “26”—day of angiogenesis assessment; except for 67NR/0: the day when tumor reached the volume of 1000 mm3, comparable to 67NR and 67NR/RBP4 on day 26; ort.—orthotopically; i.v.—intravenously.