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. 2020 Mar 16;21(6):2038. doi: 10.3390/ijms21062038

Figure 2.

Figure 2

Primary bone marrow-derived macrophages (BMDM) release Lcn-2-bound iron upon IL-10 treatment. (A) Schematic representation for the generation of conditioned media from BMDMs. (B) qRT-PCR analysis of IL-10-stimulated macrophages compared to untreated control relative to the housekeeping gene TBP (n = 5; t-test). (C) Total iron amount in the supernatant of both wt and Lcn-2−/− BMDM measured by atomic absorption spectrometry (AAS). (D) Measurement of Lcn-2 protein in macrophage conditioned media via ELISA. (E) Immunoprecipitation of Lcn-2 with subsequent iron measurements via AAS to determine the amount of Lcn-2-bound iron in macrophage conditioned media. * p < 0.05, ** p < 0.01, *** p < 0.001 (n = 5; t-test).