Figure 5.

GsMAS1 enhanced the resistance of Arabidopsis plants to Al stress (a) The phenotypes of GsMAS1 transgenic lines tolerant to Al stress. (b) Statistical analysis of relative root elongation. (c) The determination of free proline content. WT: wild type of Arabidopsis (Col-0); L4, L9, L13: GsMAS1 overexpression transgenic lines of T₃ generations. The two-day seedlings after seed germination were transferred to culture medium containing AlCl₃ (pH 4.3, 0.5 mM CaCl₂) at 0, 50, 100, 150, and 200 µM, individually. After being cultured for seven days, phenotypic images and contents of free proline for the GsMAS1 transgenic lines were recorded and/or determined for statistical analysis. Data were represented as the means ± SE of three biological replicates (Student’s test, ** p = 0.05). The programs of Image J, SPSS20, and EXCEL2000 were used to measure and/or analyze the data.