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. 2020 Mar 23;21(6):2203. doi: 10.3390/ijms21062203

Figure 6.

Figure 6

SUMOylation is necessary for Hsp90 dissociation from the ERK5-Cdc37 complex after MEK5 activation. HEK293T cells were transfected with GST-tagged ERK5 (wild type or SUMO-deficient K6/22R mutant) alone or in combination with Hsp90 and MEK5DD. Thirty-six hours later, cells were lysed, and GST-ERK5 or HA-Hsp90 proteins were affinity-purified using glutathione-sepharose or anti-HA agarose resins, respectively. Immune complexes were immunoblotted for ERK5 and Hsp90. Similar results were obtained in three independent experiments.