Figure 4.

After injection of the HMG-1 CRISPR/Cas9 knockout (KO) plasmid, high-mobility group box protein 1 (HMGB1) expression in the cortex and hippocampus was suppressed, as confirmed through Western blot and immunostaining. (A) Western blot showed a decrease in HMGB1 expression in bilateral common carotid artery occlusion (BCCAO) HMGB1-KO mice compared with BCCAO controls. (B) Quantification data of Western blot results. (C) Immunostaining further confirmed the Western blot finding that HMGB1 expression decreased in the cortex of BCCAO HMGB1-KO mice but not in BCCAO controls. (D) Quantification data of immunostaining image. n = 5 for each group. Data are presented as the mean + standard deviation; *p < 0.05; **p < 0.01; ***p < 0.001 with one-way analysis of variance followed by Sidak’s multiple comparison test; scale bar = 100 μm. Sh-C: sham control, Sh-HKO: sham HMGB1 KO, BC-C: BCCAO control, BC-HKO: BCCAO HMGB1 KO.