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. 2020 Mar 10;9(3):228. doi: 10.3390/antiox9030228

Figure 4.

Figure 4

ISL treatment induced the expression of autophagy-associated proteins in MDA-MB-231 cells. MDA-MB-231 cells were treated with ISL for 48 h. (a) The expression levels of mTOR and ULK1 protein were analyzed by Western blotting. The total and phosphorylated forms of (b) mTOR and (c) ULK1 were normalized to β-actin expression. The expression levels of (d) p62, (e) Beclin1, (f) LC3, and (g) Cathepsin B were also analyzed using Western blotting. MDA-MB-231 cells were used lipofectamine 3000 (Thermo Fisher Scientific) to transfect SignalSilence® SQSTM1/p62 (Cell Signaling), and treated with ISL for 48 h. The expression of (h) caspase-8 proteins were also analyzed using Western blotting. Data are represented as mean ± SEM (n = 3). * p < 0.05, ** p < 0.01 compared with the control group. # p < 0.05, ## p < 0.01 compared with the phosphorylated protein level of the control group.