Figure 5.

(A) Soluble $\alpha$-synuclein concentration measured in the supernatant after centrifuging the end product of the aggregation reactions in a non-binding surface plate in the presence of glass beads. Radius in nm and concentration in μM of the three replicates of $\alpha$-synuclein at pH 6 (control) (left), concentration in μM (middle), and radius in nm (right) of the end product of the aggregation reactions at pH 4, pH 5, pH 6, and pH 7. The three replicates per condition were combined before centrifugation (except for the control at pH 6, where each replicate sample was analyzed separately; see (A). (B) Amount of protein measurable with the Fluidity One (F1) MDS instrument (supernatant + pellet) in μg in the end-product of $\alpha$-synuclein at pH 7 in a non-binding surface plate without additional glass beads (left). The dotted line indicates the used amount of protein. AFM height images of the control (black frame), $\alpha$-synuclein with EGCG (1:1) (cyan frame) and of $\alpha$-synuclein with EGCG_ox (1:5) (magenta frame). The image scale is 5 × 5 μM. The color range represents the height from −2 to 10 nm (left and middle) and −10 to 25 nm (right).