Figure 1.

Enforced expression of uL3 affects rRNA processing. (A) Schematic representation of rRNA maturation process. Cleavage sites are indicated with white arrows. (B) Total RNA from HCT 116^p53−/− cells transfected or not with 1 µg of pHA-uL3 and uL3ΔHCT 116^p53−/− cells was subjected to RT-qPCR with primers specific for intermediates and mature rRNAs (Table 1). Quantification of signals is shown. Bars represent the mean of triplicate experiments; error bars represent the standard deviation. Untreated cells was set at 1. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HCT 116^p53−/− cells set at 1.