Figure 7.

Effect of uL3 restoration in uL3ΔHCT 116^p53−/− cells on cell cycle and apoptosis. (A) HCT 116^p53−/− and uL3ΔHCT 116^p53−/− cells were transiently transfected with 1 µg pHA-uL3 or treated with 25 µM CQ. 24 h later, cells were stained with PI and analysed using FACS. Peaks representing histograms of cell numbers and percentages in G1, S, and G2/M phases are shown. (B) Representative flow cytometry dot blots with double Annexin V-Alexa Fluor 488/PI staining for HCT 116^p53−/− and uL3ΔHCT 116^p53−/− transiently transfected with pHA-uL3 or treated with CQ for 24 h.