Table 5.
Candidate genes identified within 15 kb distance of the SNP markers identified for association with the traits of heat tolerance.
| Traita | SNP Marker | Gene_ID | Protein Names | Gene Names | Organismb | Gene Ontology IDs | Gene Ontology (GO) |
|---|---|---|---|---|---|---|---|
| SPAD | chr5LG3_446272814 | Psat5g221440 | Amidohydrolase ytcj-like protein (Fragment) | L195_g035501 | Tp | GO:0016810 | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds [GO:0016810] |
| chr5LG3_449362407 | Psat5g224400 | cysteine-rich receptor-like protein kinase 25 | LOC101505680 | Ca | GO:0004672; GO:0005524; GO:0016021 | integral component of membrane [GO:0016021]; ATP binding [GO:0005524]; protein kinase activity [GO:0004672] | |
| Psat5g224360 | Pentatricopeptide repeat-containing protein at1g11290-like protein | L195_g006458 | Tp | GO:0008270 | zinc ion binding [GO:0008270] | ||
| Psat5g224280 | Pentatricopeptide repeat-containing protein at1g11290-like protein | L195_g022714 | Tp | GO:0008270 | zinc ion binding [GO:0008270] | ||
| chr5LG3_566189589 | Psat5g299080 | Kinesin-related protein 4-like | L195_g011972 | Tp | |||
| Psat5g299040 | PPR containing plant-like protein (Putative tetratricopeptide-like helical domain-containing protein) | 11431556 MTR_2g102210 MtrunA17_Chr2g0331911 | Mt (Mtr) | ||||
| chr5LG3_569788697 | Psat5g301440 | Embryo-specific 3 (Fragment) | L195_g051812 | Tp | |||
| Psat5g301400 | Nuclear pore protein | LOC101492584 | Ca | GO:0005643; GO:0015031; GO:0016020; GO:0017056; GO:0051028 | membrane [GO:0016020]; nuclear pore [GO:0005643]; structural constituent of nuclear pore [GO:0017056]; mRNA transport [GO:0051028]; protein transport [GO:0015031] | ||
| chr5LG3_572899434 | Psat5g303880 | Putative sterile alpha motif/pointed domain-containing protein (SAM domain protein) | 11433470 MTR_2g102140 MtrunA17_Chr2g0331871 | Mt (Mtr) | GO:0045892 | negative regulation of transcription, DNA-templated [GO:0045892] | |
| Psat5g303840 | putative gamma-glutamylcyclotransferase At3g02910 | LOC101506022 | Ca | GO:0016740; GO:0061929 | gamma-glutamylaminecyclotransferase activity [GO:0061929]; transferase activity [GO:0016740] | ||
| Psat5g303800 | protein NUCLEAR FUSION DEFECTIVE 4 | LOC101504533 | Ca | GO:0016021 | integral component of membrane [GO:0016021] | ||
| Psat5g303760 | Uncharacterized protein | L195_g009520 | Tp | ||||
| PRI | chr6LG2_469101917 | Psat6g234040 | Putative GTP 3′,8-cyclase (EC 4.1.99.22) | MtrunA17_Chr1g0212051 | Mt (Mtr) | GO:0006777 | Mo-molybdopterin cofactor biosynthetic process [GO:0006777] |
| Psat6g234000 | Riboflavin biosynthesis protein ribF | L195_g000443 | Tp | GO:0003919; GO:0009231 | FMN adenylyltransferase activity [GO:0003919]; riboflavin biosynthetic process [GO:0009231] | ||
| chr7LG7_263964018 | Psat7g148080 | TATA-binding-like protein | L195_g000140 | Tp | GO:0005524 | ATP binding [GO:0005524] | |
| CT | chr4LG4_415994869 | Psat4g203800 | ethylene-responsive transcription factor-like protein At4g13040 | LOC105851094 | Ca | GO:0003677; GO:0003700; GO:0005634 | nucleus [GO:0005634]; DNA binding [GO:0003677]; DNA-binding transcription factor activity [GO:0003700] |
| Psat4g203760 | NA | NA | NA | NA | NA | ||
| chr5LG3_309595819 | Psat5g169800 | ABC transporter C family member 3-like isoform X1 | LOC101491790 | Ca | GO:0005524; GO:0016021; GO:0042626 | integral component of membrane [GO:0016021]; ATP binding [GO:0005524]; ATPase activity, coupled to transmembrane movement of substances [GO:0042626] | |
| Psat5g169760 | Retrovirus-related Pol polyprotein from transposon TNT 1-94 | KK1_037587 | Cc (Ci) | GO:0000943; GO:0003676; GO:0015074 | retrotransposon nucleocapsid [GO:0000943]; nucleic acid binding [GO:0003676]; DNA integration [GO:0015074] | ||
| RSL | chr3LG5_18678117 | Psat3g006600 | uncharacterized protein LOC101515092 | LOC101515092 | Ca | GO:0016021 | integral component of membrane [GO:0016021] |
| Psat3g006560 | L-allo-threonine aldolase-like protein (Putative aldehyde-lyase) (EC 4.1.2.-) | 25499717 MTR_7g115690 MtrunA17_Chr7g0274621 | Mt (Mtr) | GO:0006520; GO:0016829 | lyase activity [GO:0016829]; cellular amino acid metabolic process [GO:0006520] | ||
| chr4LG4_29062302 | Psat4g020520 | Alkaline-phosphatase-like protein (Putative Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase) | 25494146 MTR_4g123557 MtrunA17_Chr4g0069621 | Mt (Mtr) | GO:0006506; GO:0016021; GO:0051377 | integral component of membrane [GO:0016021]; mannose-ethanolamine phosphotransferase activity [GO:0051377]; GPI anchor biosynthetic process [GO:0006506] | |
| chr5LG3_566189271 | Psat5g299080 | Kinesin-related protein 4-like | L195_g011972 | Tp | |||
| Psat5g299040 | PPR containing plant-like protein (Putative tetratricopeptide-like helical domain-containing protein) | 11431556 MTR_2g102210 MtrunA17_Chr2g0331911 | Mt (Mtr) | ||||
| chr5LG3_572669963 | Psat5g303680 | Putative sterile alpha motif/pointed domain-containing protein (SAM domain protein) | 11430703 MTR_2g104230 MtrunA17_Chr2g0333351 | Mt (Mtr) | |||
| chr7LG7_20086906 | Psat7g013080 | aldehyde dehydrogenase family 2 member C4-like | LOC101493969 | Ca | GO:0016620 | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor [GO:0016620] | |
| Psat7g013040 | Cst complex subunit ctc1-like protein | L195_g004297 | Tp | GO:0000723 | telomere maintenance [GO:0000723] | ||
| chr7LG7_23295474 | Psat7g015240 | Ribosomal L7Ae/L30e/S12e/Gadd45 family protein | L195_g030323 | Tp | |||
| Psat7g015200 | Tesmin/TSO1-like CXC domain protein | 11408106 MTR_8g103320 | Mt (Mtr) | ||||
| Psat7g015160 | NA | NA | NA | NA | NA | ||
| chr7LG7_96157380 | Psat7g057080 | tRNA (Cytosine(34)-C(5))-methyltransferase-like protein | 25501876 MTR_8g089980 | Mt (Mtr) | GO:0003723; GO:0016428 | RNA binding [GO:0003723]; tRNA (cytosine-5-)-methyltransferase activity [GO:0016428] | |
| Psat7g057040 | tRNA (Cytosine(34)-C(5))-methyltransferase-like protein | 25501876 MTR_8g089980 | Mt (Mtr) | GO:0003723; GO:0016428 | RNA binding [GO:0003723]; tRNA (cytosine-5-)-methyltransferase activity [GO:0016428] | ||
| IL | chr4LG4_63111072 | Psat4g039600 | Eukaryotic translation initiation factor 3 subunit C (eIF3c) (Eukaryotic translation initiation factor 3 subunit 8) (eIF3 p110) | LOC101499912 | Ca | GO:0001732; GO:0003743; GO:0005852; GO:0016282; GO:0031369; GO:0033290 | eukaryotic 43S preinitiation complex [GO:0016282]; eukaryotic 48S preinitiation complex [GO:0033290]; eukaryotic translation initiation factor 3 complex [GO:0005852]; translation initiation factor activity [GO:0003743]; translation initiation factor binding [GO:0031369]; formation of cytoplasmic translation initiation complex [GO:0001732] |
| chr4LG4_80759704 | Psat4g047680 | NA | NA | NA | NA | NA | |
| Psat4g047640 | Ras GTPase-activating protein-binding protein 1-like | L195_g006539 | Tp | GO:0003723 | RNA binding [GO:0003723] | ||
| Psat4g047600 | Uncharacterized protein | L195_g056003 | Tp | GO:0005739 | mitochondrion [GO:0005739] | ||
| chr5LG3_566189271 | Psat5g299080 | Kinesin-related protein 4-like | L195_g011972 | Tp | |||
| Psat5g299040 | PPR containing plant-like protein (Putative tetratricopeptide-like helical domain-containing protein) | 11431556 MTR_2g102210 MtrunA17_Chr2g0331911 | Mt (Mtr) | ||||
| chr6LG2_420562729 | Psat6g211160 | Transmembrane amino acid transporter family protein | 25485307 MTR_1g105980 | Mt (Mtr) | GO:0016021 | integral component of membrane [GO:0016021] | |
| chr7LG7_197862543 | Psat7g120120 | Uncharacterized protein | 11443456 MTR_4g087360 MtrunA17_Chr4g0045601 | Mt (Mtr) | |||
| PN | chr2LG1_105547608 | Psat2g060680 | Uncharacterized protein | L195_g033306 | Tp | GO:0003676; GO:0008270 | nucleic acid binding [GO:0003676]; zinc ion binding [GO:0008270] |
| chr2LG1_370541780 | Psat2g144160 | Pectin acetylesterase (EC 3.1.1.-) | LOC101497691 | Ca | GO:0005576; GO:0005618; GO:0016021; GO:0016787; GO:0071555 | cell wall [GO:0005618]; extracellular region [GO:0005576]; integral component of membrane [GO:0016021]; hydrolase activity [GO:0016787]; cell wall organization [GO:0071555] | |
| chr2LG1_385949935 | Psat2g155280 | 60S ribosomal protein l8-like | L195_g013966 | Tp | GO:0003735; GO:0005840; GO:0006412 | ribosome [GO:0005840]; structural constituent of ribosome [GO:0003735]; translation [GO:0006412] | |
| chr2LG1_389336188 | Psat2g157440 | Putative ATPase, AAA-type, core, AAA-type ATPase domain-containing protein (p-loop nucleoside triphosphate hydrolase superfamily protein) | 11412855 MTR_5g020990 MtrunA17_Chr5g0404661 | Mt (Mtr) | GO:0005524; GO:0016787 | ATP binding [GO:0005524]; hydrolase activity [GO:0016787] | |
| chr2LG1_402022079 | Psat2g166600 | probable serine/threonine-protein kinase At1g01540 isoform X1 | LOC101489894 | Ca | GO:0004672; GO:0005524; GO:0016021 | integral component of membrane [GO:0016021]; ATP binding [GO:0005524]; protein kinase activity [GO:0004672] | |
| Psat2g166560 | PI-PLC X domain-containing protein At5g67130 | LOC101489369 | Ca | GO:0006629; GO:0008081 | phosphoric diester hydrolase activity [GO:0008081]; lipid metabolic process [GO:0006629] | ||
| Psat2g166520 | Putative rapid ALkalinization Factor (RALF) | 11409897 MTR_5g017160 MtrunA17_Chr5g0402121 | Mt (Mtr) | ||||
| chr2LG1_4359822 | Psat2g005000 | Nup133/Nup155-like nucleoporin | 11434873 MTR_5g097260 | Mt (Mtr) | GO:0005623; GO:0017056 | cell [GO:0005623]; structural constituent of nuclear pore [GO:0017056] | |
| Psat2g004960 | Cation-transporting ATPase plant (Putative calcium-transporting ATPase) (EC 3.6.3.8) | 11434874 MTR_5g097270 MtrunA17_Chr5g0447521 | Mt (Mtr) | GO:0000166; GO:0016021 | integral component of membrane [GO:0016021]; nucleotide binding [GO:0000166] | ||
| chr3LG5_216337201 | Psat3g111000 | Phosphomannomutase (EC 5.4.2.8) | 11436930 MTR_7g076670 | Mt (Mtr) | GO:0004615; GO:0005737; GO:0009298 | cytoplasm [GO:0005737]; phosphomannomutase activity [GO:0004615]; GDP-mannose biosynthetic process [GO:0009298] | |
| Psat3g110960 | bifunctional protein FolD 4, chloroplastic | LOC101496397 | Ca | GO:0004488 | methylenetetrahydrofolate dehydrogenase (NADP+) activity [GO:0004488] | ||
| chr5LG3_530537682 | Psat5g270480 | Heat shock protein 70 (HSP70)-interacting protein, putative | 25487616 MTR_2g090135 | Mt (Mtr) |
Note: The pea genome sequence reported by Kreplak et al. [40] was used for identification of candidate genes. The reported gene annotation and nomenclature was followed. a SPAD, soil plant analysis development; PRI, photochemical reflectance index; CT, canopy temperature; RSL, reproductive stem length; IL, internode length; PN, pod number; b Tp, Trifolium pratense (Red clover); Ca, Cicer arietinum (Chickpea) (Garbanzo); Mt, Medicago truncatula (Barrel medic); Mtr, Medicago tribuloides; Cc, Cajanus cajan (Pigeon pea); Ci, Cajanus indicus.