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. 2020 Mar 12;21(6):1952. doi: 10.3390/ijms21061952

Figure 2.

Figure 2

14 most active ASO candidates against TGFBR2 after gymnotic delivery. Remaining TGFBR2 mRNA after 72 h of incubation with different ASOs as measured in a bDNA assay (n = 4) relative to the reference gene GAPDH. TGFBR2 expression was normalized to respective PBS-treated control cells. Panc-1 cells were treated with 5 µM, A549 and 4T1 cells with 7.5 µM of each ASO. Statistics was calculated by Ordinary-one-way-Anova followed by Dunnett’s multiple comparison test. ** p ≤ 0.0, ± = SEM. XO = ASOs, R14082 ASO as negative control directed against Aha-4 with readout for remaining TGFBR2 mRNA. Aha-1 ASO as positive control with readout for remaining Aha-1 mRNA to determine a successful assay-performance.