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Used antibodies for immunocytochemistry.
| A549 cells | |||
|---|---|---|---|
| Primary Antibody | Dilution | Company | Order Number |
| FN (rabbit) | 1:50 | Proteintech (St. Leon-Rot, Germany) | 15613-1-AP |
| pSmad2 (rabbit) | 1:50 | Cell Signaling (Danvers, MA, USA) | cs3104s |
| TGF-βRII (rabbit) | 1:50 | Millipore (Darmstadt, Germany) | 06-227 |
| Secondary Antibody | Dilution | Company | Order Number |
| Cy3 goat-anti-rabbit | 1:1000 | Life Technologies (Darmstadt, Germany) | A10520 |
| Alexa Fluor 488 | 1:1500 | Life Technologies (Darmstadt, Germany) | A21441 |
