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. 2020 Mar 19;9(3):251. doi: 10.3390/antiox9030251

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Interleukin-6 induces human heme oxygenase-1 gene expression. HepG2 cells were transiently transfected with pcDNA3zeo-IL-6 (HepG2-IL-6) or pcDNA3.1zeo (HepG2–DNA) expression vectors. The expressions of HO-1 and IL-6 were analyzed using ELISA (A), RT-qPCR (B), and immunoblot (C) assays. (D) HepG2 cells were treated with recombinant human IL-6 (10 ng/mL) at various times (top), or with various concentrations of IL-6 as indicated for 16 h (bottom). (E) HepG2 cells were treated with IL-6 (10 ng/mL), or IL-6 co-treated with/without an anti-human IL-6 monoclonal antibody (IL-6MAB) for 16 h. Expressions of HO-1 protein levels were analyzed using immunoblot assays. (**, p < 0.01).