Skip to main content
. 2020 Mar 19;9(3):251. doi: 10.3390/antiox9030251

Figure 5.

Figure 5

Activation of Interleukin-6 on reporter activity of human heme oxygenase-1 gene is dependent on STAT3 expression. (A) Nested deletion constructs of heme oxygenase-1 reporter vectors. (B) The reporter activities of the HO-1 reporter vectors and the reporter vectors containing with (ppGL3) or without (pbGL3) SV40 promoter. (C) The HO-1 reporter vector (pHO1-KX) was co-transfected with different concentrations of the IL-6 expression vector into HepG2 cells. (D) The relative luciferase activities of reporter vectors, containing different fragments from the HO-1 enhancer/promoter, were co-transfected with the IL-6 expression vector (0.2 μg/well) into HepG2 cells. (E) The relative luciferase activities of STAT3 reporter vector-transfected HepG2 cells were co-transfected with IL-6 (pcDNA-IL-6), STAT3 (pcDNA-STAT3) and/or PIAS3 (pcDNA-PIAS3) expression vectors. (F) The HO-1 reporter vector (pHO1-KX) was transiently co-transfected with IL-6 expression, STAT3 expression and/or PIAS3 expression vectors into HepG2 cells. Data are expressed as mean ± SE (n = 6) of luciferase activity relative to the control-treated group or to the group as indicated. (*, p < 0.05; **, p < 0.01).