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. 2020 Mar 7;9(3):222. doi: 10.3390/antiox9030222

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Relative gene expression by real-time PCR of genes involved in AsA biosynthesis. For each genotype (M82, IL7-3 and IL12-4), quantities (RQ) are relative to the calibrator condition (fruit explant) and are graphed on a logarithmic scale. For each gene, asterisks indicate that the 2^−ΔCt values were significantly different between the test (calli at 60 days) and calibrator condition (** p < 0.01; t-test).

Relative gene expression by real-time PCR of genes involved in AsA biosynthesis. For each genotype (M82, IL7-3 and IL12-4), quantities (RQ) are relative to the calibrator condition (fruit explant) and are graphed on a logarithmic scale. For each gene, asterisks indicate that the 2^−ΔCt values were significantly different between the test (calli at 60 days) and calibrator condition (** p < 0.01; t-test).