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. 2020 Mar 11;21(6):1927. doi: 10.3390/ijms21061927

Figure 5.

Figure 5

Decreased CD44 expression in spheroids by NEAT1 deficiency. (A,B) mRNA expression levels relative to HPRT1 in spheroids of HuH7 (A) and HepG2 (B) cells. White columns, parental cells (WT); gray and black columns, NEAT1-KO cells. * p < 0.05 vs. WT; Dunnett’s test (HuH7) or Student’s t-test (HepG2) (n = 3). (C) CD44 protein expression in monolayer cells (2D) and in spheroids (3D) of HuH7 (left and middle) and HepG2 (right). WT, parental cells; KO, NEAT1-KO cells. GAPDH was used as an internal control.