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qRT-PCR for RNAseq data validation of genes involved with plant defense responses. Comparison between NPR1-2 transgenic and non-transgenic plants of genes involved in the (A) NPR1-SA dependent pathway, including CsNPR1, CsNPR3, and CsSABP2 along with pathogenesis related proteins, CsPR1 and CsPR2; and (B) transcription factors. Different letters (a, b) represent a significant difference at p ≤ 0.05 using Duncan’s Multiple Range Test and error bars represent SE (n = 3).
