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Knockdown of the ACSL1 gene in sheep preadipocytes: (a) the pGPU6 vector; (b) ACSL1 mRNA expression on day 8 of differentiation (** p < 0.01); (c) COX2 mRNA expression on day 8 of differentiation (** p < 0.01); (d) ACSL1 and COX2 protein expression on day 8 of differentiation (** p < 0.01). shNC: Adipocytes transfected with shNC as control. shACSL1: Adipocytes transfected with shRNA-1928 of the ACSL1 gene.
