Figure 1.

Reactive gliosis increases in the old hippocampus. We determined the hippocampal tissue integrity by hematoxylin–eosin staining and no detection of protein inclusions, cells with apoptotic characteristics, or other abnormalities (A). We observed a significant increase of the glial fibrillary acidic protein (GFAP) protein level in the astrocytes from the elderly vs. the young brain (B). Values are mean ± SEM; Statistics were performed by unpaired t-test. Young (n = 7): 20–30 years old. Elder (n = 6): >60 years old. Scale bar = 100 μm.