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. 2020 Mar 23;21(6):2215. doi: 10.3390/ijms21062215

Figure 2.

Figure 2

IL-17A does not induce cancer stem cell features in human or mouse PDAC cells in vitro. (a) Wound healing assay in pancreatic cancer cell lines. MIA PaCa-2 and Panc02 were treated 48 h with 20 ng/mL recombinant IL-17A or PBS. The wound area was assessed 24 and 48 h after scratching. The boundary of cancer cell migration is marked with red lines. (b) Sphere formation assay using human L3.6pl cells treated with 20 ng/mL recombinant human IL-17A or PBS for 48 h. Sphere formation rate at day 7 was calculated as the sphere number divided by the initial cell number plated and expressed as a percentage (Mean ± SEM). (c) Detection of human pancreatic stem cell markers ABCG2 and CXCR4 using flow cytometry in human pancreatic L3.6pl cells treated with 20 ng/mL recombinant human IL-17A or PBS for 48 h. CD44 was assessed in the pancreatic mouse cell line Panc02 under the same experimental setup.