Figure 3.

PDT-based DC vaccination promotes CD8⁺ T cell response. The CD8⁺ splenocytes collected at day 55 post-treatment (i.e., PDT-based DC vaccine or anti-CTLA4 antibodies, see Figure 2E) were re-exposed to the Ab1 mesothelioma cells. (A) The proliferation of CD8⁺ splenocytes was detected by CFSE dilution (n = 3, * p < 0.05, *** p < 0.001; ns = non-significant). (B) The survival of Ab1 mesothelioma cells, as determined four days after exposure to CD8⁺ cells (n = 3, *** p < 0.001; ns = non-significant). (C) IFN-γ production and (D) LAMP-1 staining were detected 18 h after Ab1 cell re-exposure (n = 3, ** p < 0.01, *** p < 0.001; ns = non-significant).